一、基本信息
郭瑞庭，二级教授、博士研究生导师
电子邮件：guoreyting(at)hubu.edu.cn
研究方向：酶工程、结构生物学与酶学
研究领域：酶分子结构、机理研究、应用与改造
郭瑞庭教授通过结构生物学方法解析重要工业酶结构，阐释催化机理，进行酶分子理性设计，以改造酶的活性与特性。工作内容主要涵盖五个体系：(1) PET塑料降解酶；(2)新型萜类合成酶；(3)霉菌毒素分解酶；(4)生物质降解酶；(5) P450酶。目前已在Nature Reviews Chemistry, Nature Catalysis, Immunity, Nature Communications, Angew. Chem. Intl. Ed., JACS, ACS Catalysis, PNAS等SCI期刊发表论文140余篇，超过30篇获选为封面文章，申请国内外专利34个，已授权23个。2019年获天津市自然科学二等奖(第一完成人)。
二、教育背景
2003–2007 台湾大学生化科学所暨台湾中研院生物化学所，博士
2014–2017 台湾政治大学EMBA，获商学硕士
三、工作经历
2018年至今 湖北大学生命科学院、省部共建生物催化与酶工程国家重点实验室，二级教授/博导
2010–2018 中科院天津工业生物技术研究所，研究员、博导
2007–2017 年台湾基酵生物科技公司，研发总监/研发总顾问
2007–2009 美国The Scripps Research Institute，博士后研究
四、科研项目
1.国家重大专项、2019.12–2024.11，552万、在研、主持
2.湖北省创新群体、2020.03–2023.03，50万、在研、主持
3.自然科学基金面上项目（两次），2015.001–2018.012、2019.001-2022.012、85万、在研、主持
4.863计划、2012.8-2015.12、项目9450万、已结题、项目首席科学家
5.973计划、2011.01-2015.08、75万、已结题、主持
6.中科院创新交叉团队、2017.01-2019.12、100万、已结题、团队负责人
7.中科院重点部署项目，2013.01-2015.12、235万、已结题、主持
8.中科院百人计划、2010年-2013年、260万、已结题、主持
9.天津市科技项目、2011年-2014年、150万、已结题、主持
10.企业合作开发项目(东莞泛亚太)、2012年-2015年、255万、已结题、主持
四、代表性论文
1.Zhang, X.,#Shen, P.,#Zhao, J.,#Chen, Y., Li, X., Huang, J.-W., Zhang, L., Li, Q., Gao, C., Xing, Q., Chen, C.-C.,Guo, R.-T.,*Li, A.*. Rationally controlling selective steroid hydroxylation via scaffold sampling of a P450 Family.ACS Catalysis,2023, 13, 1280–1289.
2.Chen, Q.,#Li, B.,#Zhang, L.,#Chen, X., Zhu, X., Chen, F., Shi, M., Chen, C.-C., Yang, Y.,Guo, R.-T.,*Liu, W., Xu, J., Zheng, G.*. Engineered imine reductase for larotrectinib intermediate manufacture.ACS Catalysis,2022, 12 (23), 14795-14803.
3.Dai, L.,#Li, H.,#Huang, J.-W.,#Hu, Y.,#He, M., Yang, Y., Min, J.,Guo, R.-T.,*Chen, C.-C.*. Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin.International Journal of Biological Macromolecules,2022-12-01, 222: 421-428.
4.Wu, L.,#An, J.,#Jing, X.,#Chen, C.-C., Dai, L., Liu, W.,*Guo, R.-T.,*Nie, Y.*. Molecular insights into the regioselectivity of the Fe(II)/2-ketoglutarate-dependent dioxygenase-catalyzed C-H hydroxylation of amino acids.ACS Catalysis,2022-09-08, 12: 11586-11596.
5.Zhang, L.,#Zhang, X.,#Min, J.,#Liu, B., Huang, J.-W., Yang, Y., Liu, W., Dai, L., Yang, Y., Chen, C.-C.,*Guo, R.-T.*.. Structural insights to a bi-functional isoprenyl dihposphate synthase that can catalyze head-to-tail and head-to-middle condensation.International Journal of Biological Macromolecules,2022-08-01, 214: 492-499.
6.Li, H.,#Yang, Y.,#Hu, Y.,#Chen, C.-C., Huang, J.-W., Min, J., Dai, L.,*Guo, R.-T.*.Structural analysis and engineering of aldo-keto reductase from glyphosate-resistantEchinochloa colona.Journal of Hazardous Materials, 2022-08-15, 436: 129191.
7.Malwal, S. R.,#Shang, N.,#Liu, W., Li, X., Zhang, L., Chen, C.-C.,Guo, R.-T.,*Oldfield, E.*. A structural and bioinformatics investigation of a fungal squanlene synthase and comparisons with other membrane proteins.ACS Omega,2022-06-17, 7 (26): 22601-22612.
8.Wang, T.,#Yang, Y.,#He, M., Liu, M., Huang, J.-W., Min, J., Chen, C.-C., Liu, Y.,* Zhang, L.,*Guo, R.-T.*.Structural insights into the cyclization of unusual brasilane-type sesquiterpenes.International Journal of Biological Macromolecules,2022-04-30, 209: 1784-1791.
9.Dai, L.,#Zhang, X.,#Hu, Y.,#Shen, J., Zhang, Q., Zhang, L., Min, J., Chen, C.-C., Liu, Y.,* Huang, J.-W.,*Guo, R.-T.*.Structural and functional insights into a nonheme iron- and α-ketoglutarate-dependent halogenase that catalyzes chlorination of nucleotide substrates.Applied and Environmental Microbiology, 2022-04-18, 88 (9): e0249721.
10.Zeng, W.,#Li, X.,#Yang, Y.,#Min, J.,#Huang, J.-W., Liu, W., Niu, D., Yang, X., Han, X., Zhang, L., Dai, L., Chen, C.-C.,*Guo, R.-T.*.Substrate-binding mode of a thermophilic PET hydrolase and engineering the enzyme to enhance the hydrolytic efficacy,ACS Catalysis, 2022-02-18, 12: 3033-3040.
11.Chen, C.-C.,#Dai, M.,#Zhang, L.,#Zhao, J., Zeng, W., Shi, M., Huang, J.-W., Liu, W.,Guo, R.-T.,*Li, A.*. Molecular basis for a toluene monooxygenase to govern substrate selectivity,ACS Catalysis, 2022-02-13, 12: 2831-2839.
12.Mao, Z.,#Xiao, H.,#Shen, P.,#Yang,Y.,#Xue, J., Yang, Y., Shang, Y., Zhang, L., Li, X., Zhang, Y., Du, Y., Chen, C.-C.,Guo, R.-T.,*Zhang, Y.*. KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge.Cell Discovery,2022-01-25, 8 (1): 5.
13.Hu, Y.#, Li, H.,#Min, J.,#Yu, Y., Liu, W., Huang, J.-W., Zhang, L., Yang, Y., Dai, L.*, Chen, C.-C.,*Guo, R.-T.*.Crystal structure and biochemical analysis of the specialized deoxynivalenol–detoxifying glyoxalase SPG fromGossypium hirsutum.International Journal of Biological Macromolecules, 2022-01-18, 200: 388-396.
14.陈纯琪,韩旭,刘卫东,马立新,刘珂*，郭瑞庭*.基于结构改造来源于大阪伊德氏杆菌201-F6的PET水解酶，生物工程学报，2021-09-25, 37 (9): 3268-3275.
15.Dai, L.,#Chang, Z.,#Yang, J., Liu,W., Yang, Y., Chen, C.-C., Zhang, L., Huang, J.-W.,* Sun, Y.,*Guo, R.-T.*.Structural investigation of a thermostable 1,2- β -mannobiose phosphorylase fromThermoanaerobactersp.X-514.Biochemical and Biophysical Research Communications, 2021-09-21, 579: 54-61.
16.Dai, L.,#Qu, Y.,#Hu, Y.,#Min, J., Yu, X., Chen, C.-C., Huang, J.-W.,*Guo, R.-T.*.Catalytically inactive lytic polysaccharide monooxygenase PcAA14A enhances the enzyme-mediated hydrolysis of polyethylene terephthalate,International Journal of Biological Macromolecules,2021-09-07, 190: 456-462.
17.Dai, L.,#Qu, Y.,#Huang, J.-W., Hu, Y., Hu, H., Li, S., Chen, C.-C.,*Guo, R.-T.*.Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I fromTrichoderma reesei.Journal of Biotechnology,2021-05-24, 334: 47-50.
18.Liu, W.,#Ma, C.,#Liu, W.,#Zheng, Y.,Chen, C.-C., Liang, A., Luo, X., Li, Z., Ma, W., Song, Y.,*Guo, R.-T.,*Zhang, T.*. Functional and structural investigation of a novel β-mannanase BaMan113A fromBacillussp.N16-5.International Journal of Biological Macromolecules, 2021-04-15, 182: 899-909.
19.Chen, C.-C.,#Han, X.,#Li, X.,#Jiang, P., Niu, D., Ma, L., Liu, W., Li, S., Qu, Y., Hu, H., Min, J., Yang, Y., Zhang, L., Zeng, W., Huang, J.-W.,* Dai, L.,*Guo, R.-T.*General features to enhance enzymatic activity of poly (ethylene terephthalate) hydrolysis,Nature Catalysis,2021,4, 425-430(Highlighted by ChemistryViews,科技日报,中国科学报)
20.Dai, L., Hu, Y.,Chen, C.-C., Ma, L.,*Guo, R.-T.*Flavonoid C-glycosyltransferases: function, evolutionary relationship, catalytic mechanism and protein engineering,ChemBioEng Reviews, 2021-01-20, 21 (1): 15-26.
21.Chen, C.-C.,#Yu, X.,#Kuo, C.-J.,#Min, J., Chen, S., Ma, L.,* Liu, K.,* Guo, R.-T.*Overview of antiviral drug candidates targeting coronaviral 3C-like main proteases.FEBS Journal,2021, (Review,https://doi.org/10.1111/febs.15696)
22.Chen, C.-C., Min, J., Zhang, L., Yang, Y., Yu, X.,* Guo, R.-T.*Advanced Understanding of the Electron Transfer Pathway of Cytochrome P450s.ChemBioChem,2021,22, 1317-1328(Review,Coverstory)
23.Chen, C.-C.,#Malwal, S. R.,#Han, X.,#Liu, W., Ma, L., Zhai, C., Dai, L., Huang, J.-W., Shillo, A., Desai, J., Ma, X., Zhang, Y.,Guo, R.-T.,*Oldfield, E.*Terpene Cyclases and Prenyltransferases: Structures and Mechanisms of Action.ACS Catalysis,2021,11, 290-303 (Cover story)
24.Chen, C.-C.,#Jian Min, J.,#Zhang, L., Yang, Y., Yu, X.,*Guo, R.-T.*Advanced understanding of electron transfer pathway of cytochrome P450s,ChemBioChem，2021-07-05,22: 1317-1328.(Review,Coverstory)
25.Chen, C.-C., Xue, J., Peng, W., Wang, B., Zhang, L., Liu, W., Ko, T.-P., Huang, J.-W., Zhou, S., Min, J., Ma, L., Dai, L.,*Guo, R.-T.*.Yu, X.*. Structural insights into thebaine synthase 2 catalysis.Biochemical and Biophysical Research Communications,2020-06-22, 529 (2):156-161.
26.Zhang, L.,#Xie, Z.,#Liu, Z.,#Zhou, S., Ma, L., Liu, W., Huang, J.-W., Ko, T.-P., Li, X., Hu, Y., Min, J., Yu, X.,Guo, R.-T.,*Chen, C.-C.*. Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase.Nature Communications,2020,11, 2676.(Highlighted by C&EN, ChemistryViews,科技日报,中国科学报)
27.Chen, C.-C.,* Zhang, L., Yu, X., Ma, L., Ko, T.-P.,Guo, R.-T.*.Versatile cis-isoprenyl diphosphate synthase superfamily members in catalyzing carbon–carbon bond formation.ACS Catalysis,2020,10, 4717-4725.(Review, ACS Editors’ Choice, Cover story)
28.Chen, C.-C., Dai, L., Ma, L.,*Guo, R.-T.*.Enzymatic degradation of plant biomass and synthetic polymers.Nature Reviews Chemistry,2020,4, 114-126.(Review, Cover story)
29.Song, M., Zhang, X., Liu, W.,* Feng, J.,* Cui, Y., Yao, P., Wang, M., Guo, R.-T.,Wu, Q., Zhu, D.*.2,3-Dihydroxybenzoic Acid Decarboxylase fromFusarium oxysporum: Crystal Structures and Substrate Recognition Mechanism.ChemBioChem,2021,21, 2950-2956.
30.Huang, J.-W.,#Niu, D.,#Liu, K.,#Wang, Q., Ma, L., Chen, C.-C., Zhang, L., Liu, W., Zhou, S., Min, J., Wu, S., Yang, Y.,* Guo, R.-T.*.Structure basis of non-structural protein pA151R from African Swine Fever Virus.Biochemical and Biophysical Research Communications,2020,532, 108-113.
31.Liu, Y., Lee, C., Li, F., Trček, J., Bähre, H., Guo, R.-T.,Chen, C.-C., Chernobrovkin, A., Zubarev, R., Römling, U. A Cyclic di-GMP Network Is Present in Gram-Positive Streptococcus and Gram-Negative Proteus Species.ACS Infectious Diseases,2020,6, 2672-2687.
32.Xiao, X., Elsayed, S. S., Wu, C., van der Heul, H. U., Metsä-Ketelä, M., Du, C., Prota, A. E., Chen, C.-C., Liu, W., Guo, R.-T.,Abrahams, J. P., van Wezel, G. P.Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway.ACS Chemical Biology,2020,15, 2529-2538
33.Zhou, S., Ko, T.-P.,* Huang, J.-W., Liu, W., Zheng, Y., Wu, S., Wang, Q., Xie, Z., Liu, Z., Chen, C.-C.,* Guo, R.-T.*.Structure of a gut microbial Diltiazem-metabolizing enzyme suggests possible substrate binding mode.Biochemical and Biophysical Research Communications,2020,527, 799-804.
34.Chen, X., Zhang, H., Maria-S., Miguel A., Liu, W., Li, J., Feng, J., Liu, X., Osuna S.,*Guo, R.-T.,Wu, Q.,* Zhu, D.,* Ma, Y. Efficient reductive desymmetrization of bulky 1, 3-cyclodiketones enabled by structure-guided directed evolution of a carbonyl reductase,Nature Catalysis,2019,2, 931-941.
35.Yang, Y., Li, L., Yuan, L., Zhou, X., Duan, J., Xiao, H., Cai, N., Han, S., Ma, X., Liu, W., Chen, C.-C., Wang, L., Li, X., Chen, J., Kang, N., Chen, J., Shen, Z., Malwal, S. R., Liu, W., Shi, Y., Oldfield, E.,Guo, R.-T.,*Zhang, Y.*. A structural change in butyrophilin upon phosphoantigen binding underlies phosphoantigen-mediated Vgamma9Vdelta2 T cell activation.Immunity,2019,50, 1043-1053
36.Sun, H., Ko, T.-P., Liu, W., Liu, W., Zheng, Y., Chen, C.-C.,*Guo, R.-T.*.Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate.Biochemical and Biophysical Research Communications,2019,521, 31-36.
37.Kuo, C.-J., Gao, J., Huang, J.-W., Ko, T.-P., Zhai, C., Ma, L., Liu, W., Dai, L., Chang, Y.-F., Chen, T.-H., Hu, Y., Yu, X.,Guo, R.-T.,*Chen, C.-C.*. Functional and structural investigations of fibronectin-binding protein Apa fromMycobacteriumtuberculosis.Biochimica et Biophysica Acta.General subjects,2019,1863,1351-1359
38.Tang, X.,#Xue, J.,#Yang, Y., Ko, T.-P., Chen, C.-Y., Dai, L.,Guo, R.T.,Zhang, Y.,* Chen C. C.*. Structural insights into the calcium dependence of Stig cyclases.RSC Advances,2019,9,13182.
39.Ma, J., Ko, T.-P., Yu, X., Zhang, L., Ma, L., Zhai, C.,Guo, R.-T.,Liu, W., Li, H., Chen, C.-C.*. Structural insights to heterodimericcis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1.Biochemical and Biophysical Research Communications,2019,515,621-626.
40.Zhang, L., Ko, T.-P., Malwal S. R., Liu W., Zhou S., Oldfield E.,Guo R. T.,*and Chen, C.-C.*. Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism,Biochemical and Biophysical Research Communications,2019,511, 800-805
41.Chang, Z., Ansbacher, T., Zhang, L., Yang, Y., Ko, T.-P., Zhang, G., Liu, W., Huang, J.-W., Dai, L.,Guo, R.-T.,Major, D. T.,* Chen, C.-C.*. Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis,Organic Biomolecular Chemistry,2019,17, 2070-2076.(Cover story)
42.Malwal, S. R., Chen, L., Hicks, H., Qu, F., Liu, W., Shillo, A., Law, W. X., Zhang, J., Chandnani, N., Han, X., Zheng, Y., Chen, C.-C., Guo, R.-T.,AbdelKhalek, A., Seleem, M. N., Oldfield, E.Discovery of lipophilic bisphosphonates that target bacterial cell wall and quinone biosynthesis.Journal of Medicinal Chemistry,2019,62, 2564-2581.
43.Zhang, M., Yu, X.-W., Xu, Y., Guo, R.-T.,Swapna, G. V. T, Szyperski, T., Hunt, J. F., Montelione, G. T.Structural Basis by Which the N-Terminal Polypeptide Segment of Rhizopus chinensis Lipase Regulates Its Substrate Binding Affinity.Biochemistry,2019,58, 3943-3954.
44.Chen, C.-C.,#Hu, X.,#Tang, X.,#Yang, Y.,#Ko, T.-P., Gao, J., Zheng, Y., Huang, J.-W., Yu, Z., Li, L., Han, S., Cai, N., Zhang, Y.,* Liu, W.,* Guo, R.-T.*.The crystal structure of a class of cyclases that catalyze the Cope rearrangement.Angewandte Chemie International Edition,2018,57, 15060-15064.(Cover story)
45.Gao, J.,#Ko, T.-P.,#Chen, L.,#Malwal, S. R., Zhang, J., Hu, X., Qu, F., Liu, W., Huang, J.-W., Cheng, Y.-S., Chen, C.-C., Yang, Y., Zhang, Y., Oldfield, E.,Guo, R.-T.*.“Head-to-middle” and “head-to-tail”cis-prenyl transferases: Structure of isosesquilavandulyl diphosphate synthase.Angewandte Chemie International Edition,2018,57, 683-687.(Cover story)
46.Chen, C.-C.,#Han, X.,#Ko, T.-P., Liu, W.,*Guo, R.-T.*.Structural studies reveal the molecular mechanism of PETase.FEBS Journal,2018,285, 3717-3723.
47.You, S., Chen, C.-C., Tu, T., Wang, X., Ma, R., Cai, H.-Y., Guo, R.-T.,*Luo, H.-Y.,* Yao, B.*. Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis.Biotechnol Biofuels,2018,11, 159.
48.Zheng, Y.,#Liu, W.,#Chen C. C.,#Hu, X., Liu, W., Ko, T.-P., Tang, X., Wei, H., Huang, J.-W.,Guo, R.-T.*.Crystal structure of a mycoestrogen-detoxifying lactonase from Rhinocladiella mackenziei: Molecular insight into ZHD substrate selectivity.ACS Catalysis. 2018,8, 4294-4298.
49.Malwal, S. R.,#Gao, J.,#Hu, X.,#Yang, Y.,#Liu, W., Huang, J.-W., Ko, T.-P., Li, L., Chen, C.-C., O’Dowd, B., Khade, R. L., Zhang, Y., Zhang, Y., Oldfield, E.,Guo, R.-T.*.Catalytic role of conserved asparagine, glutamine, serine, and tyrosine residues in isoprenoid biosynthesis enzymesACS Catalysis. 2018,8, 4299-4312.
50.Lee, S. M.-Y., Yip, T., Yan, S., Jin, D., Wei, H.,Guo, R.-T.,Peiris, JS Malik.*. Recognition of double-stranded RNAand regulation of interferon pathway by Toll-likereceptor10.Frontiers in Immunology.2018,9, 516.
51.Wang, J.,#Chen, C.-C.,#Yang, Y.,#Liu, W., Ko, T.-P., Shang, N., Hu, X., Xie, Y., Huang, J.-W., Zhang, Y.,*Guo, R.-T.*.Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis.Biochemical and Biophysical Research Communications, 2018,495, 1782-1788.
52.Han, X.,#Liu, W.,#Huang, J.-W.,#Ma, J., Zheng, Y., Ko, T.-P., Xu, L., Cheng, Y.-S., Chen, C.-C.,Guo, R.-T.*.Structural insight into catalytic mechanism of PET hydrolase.Nature Communications,2017,13, 2106.
53.Gao, J.,#Huang, J.-W.,#Li, Q.,#Liu, W., Ko, T.-P., Zheng, Y., Xiao, X., Kuo, C.-J., Chen, C.-C.,*Guo, R.-T.*.Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-β-endoglucanase fromThielavia terrestris.Enzyme and Microbial Technology,2017,99, 32-37.
54.Schwalen, C. J., Feng, X., Liu, W., O-Dowd, B., Ko, T.-P., Shin, C. J.,Guo, R.-T.,Mitchell, D. A.,* Oldfield, E.*. Head-to-head prenyl synthases in pathogenic bacteria.ChemBioChem : a European Journal of Chemical Biology, 2017,18, 985-991.
55.Hui, R.,#Hu, X.,#Liu, W., Zheng, Y., Chen, Y.,Guo, R.-T.,Jin, J.,* Chen, C.-C.*.Characterization and crystal structure of a novel zearalenone hydrolase fromCladophialophora bantiana.Acta Crystallographica. Section F, Structural Biology Communications, 2017,73, 515-519.
56.Zhang, L.,#Chen, C.-C.,#Ko, T.-P.,#Huang, J.-W., Zheng, Y., Liu, W., Wang, I., Malwal, S. R., Feng, X., Wang, K., Huang, C. C., Hsu, S.-T. D., Wang, A. H.-J., Oldfield, E.,* andGuo, R.-T.*.Moenomycin biosynthesis: structure and mechanism of action of the prenyl transferase MoeN5,Angewandte Chemie International Edition,2016,55, 4716-4720.
57.Xu, Z.,#Liu, W.,#Chen, C.-C.,#Li Q., Huang, J.-W., Ko, T.-P., Liu, G., Liu, W., Peng, W., Cheng, Y.-S., Chen, Y., Jin, J., Li, H.,* Zheng, Y.,*Guo, R.-T.*.Enhanced α‑zearalenol hydrolyzing activity of a mycoestrogen detoxifying lactonase by structure-based engineering.ACS Catalysis, 2016,6, 7657-7663.(Cover story)
58.Liu, M.,#Chen, C.-C.,#Chen, Lu.,#Xiao, X., Zheng, Y.,Huang, J.-W., Liu, W., Ko, T.-P., Cheng, Y.-S., Feng, X., Oldfield, E.,*Guo, R.-T.,*and Ma, Y.*. Structure and function of a “head-to-middle” prenyltransferase: Lavandulyl diphosphate Synthase,Angewandte Chemie International Edition,2016,55, 4721-4724.(Cover story)
59.Wang, Y., Desai, J., Zhang, Y., Malwal, S. R., Shin, C. J., Feng, X., Sun, H., Liu, G.,Guo, R.-T.,Oldfield, E.*. Bacterial dell growth inhibitors targeting undecaprenyl diphosphate synthase and undecaprenyl diphosphate phosphatase.ChemMedChem, 2016,11, 2311-2319.
60.Yan, J.,#Liu, W.,#Li, Y., Lai, H.-L., Zheng, Y., Huang, J.-W., Chen, C.-C., Chen, Y., Jin, J., Li, H.,*Guo, R.-T.*.Functional and structural analysis ofPichia pastorisexpressedAspergillus niger1,4-β-endoglucanase.Biochemical and Biophysical Research Communications, 2016,17, 8-12.
61.Desai, J., Liu, Y.-L., Wei, H., Liu, W., Ko, T.-P.,Guo, R.-T.,Oldfield, E.*.Structure, function, and inhibition ofStaphylococcus aureusheptaprenyl diphosphate synthase.ChemMedChem, 2016,11, 1915-1923.
62.Huang, J.-W.,#Liu, W.,#Lai, H.-L., Cheng, Y.-S., Zheng, Y., Li, Q., Sun, H., Kuo, C.-J.,Guo, R.-T.,*Chen, C.-C.*.Crystal structure and genetic modifications of FI-CMCase fromAspergillus aculeatusF-50.Biochemical and Biophysical Research Communications, 2016,16, 565-572.
63.Yang, Y.,#Ko, T.-P.,#Chen, C.-C.,#Huang, G., Zheng, Y., Liu, W., Wang, I., Ho, M., Hsu, S., O'Dowd, B., Huff, H., Huang, C.-H., Docampo, R., Oldfield, E.,*Guo, R.-T.*.Structures of trypanosome vacuolar soluble pyrophosphatases: Anti-parasitic drug targets.ACS Chemical Biology, 2016,11, 1362-1371.
64.Zheng, Y.,#Li, Y.,#Liu, W.,#Chen, C.-C., Ko, T.-P., He, M., Xu, Z., Liu, M., Luo, H.,Guo, R.-T.*.Yao, B,* and Ma, Y.* Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-β-1, 4-xylanase.Journal of Structural Biology, 2016,193, 206-211.(Cover story)
65.Liu, G., Li, Q., Shang, N., Huang, J.-W., Ko, T.-P., Liu, W., Zheng, Y., Han, X., Chen, Y., Chen, C.-C., Jin, J.,Guo, R.-T.*.Functional and structural analyses of a 1,4- β-endoglucanase fromGanoderma lucidum.Enzyme and Microbial Technology,2016,86, 67-74.
66.Hu, Y.,#Liu, W.,#Malwal, S. R.,Zheng, Y., Feng, X., Ko, T.-P., Chen, C.-C., Xu, Z., Liu, M., Han, X., Gao, J., Oldfield, E.,*Guo, R.-T.*.Structures of iridoid synthase fromCantharanthus roseuswith bound NAD(+), NADPH, or NAD(+)/10-oxogeranial: Reaction mechanisms.Angewandte Chemie International Edition,2015,54, 15478-15482.(Cover story)
67.Cheng, Y.-S., Chen, C.-C., Huang, J.-W., Ko, T.-P., Huang, Z.,Guo, R.-T.*.Improving the catalytic performance of a GH11 xylanase by rational protein engineering.Applied Microbiology and Biotechnology,2015,99, 9503-9510.
68.Zheng, Y., Chen, C.-C., Ko, T.-P., Xiao, X., Yang, Y., Huang, C.-H., Qian, G., Shao, W.,*Guo, R.-T.*.Crystal structures of S-adenosyl homocysteine hydrolase from the thermophilic bacteriumThermotoga maritima.Journal of Structural Biology, 2015,190, 135-142.(Cover story)
69.Guo, R.-T.*.Green Enzymes.ChemBioEng Reviews, 2015,2, 75.(Invited Editorial)
70.Chen, C.-C., Cheng, K. J., Ko, T.-P.,Guo, R.-T.*.Current progresses in phytase research: Three-dimensional structure and protein engineering.ChemBioEng Reviews, 2015, 2, 76-86.(Cover story)
71.Chen, C.-C., Ko, T.-P., Huang, J.-W.,Guo, R.-T.*.Heat- and alkaline-stable xylanases: applications, protein structure and engineering.ChemBioEng Reviews, 2015,2, 95-106.
72.Chan, H.-C.,#Ko, T.-P.,#Huang, C.-H.,Guo, R.-T.*.Minireview: A comeback of Hg-derivatives in protein crystallography with Cys-modification.ChemBioEng Reviews, 2015,2, 133-140.
73.Liu, W.,Guo, R.-T.,Chen, X., Li, Z., Gao, X., Huang, C.-H., Wu, Q., Feng, J., Zhu, D.*. Structural analysis reveals the substrate-binding mechanism for the expanded substrate specificity of mutantmeso-diaminopimelate dehydrogenase.ChemBioChem : a European Journal of Chemical Biology, 2015,16, 924-929.(Cover story)
74.Chen, Y., Huang, J.-W., Chen, C.-C., Lai, H.-L., Jin, J.,Guo, R.-T.*.Crystallization and preliminary X-ray diffraction analysis of an endo-1, 4-beta-D-glucanase fromAspergillus aculeatusF-50.Acta Crystallographica. Section F, Structural Biology Communications, 2015,71, 397-400.
75.Zhu, W., Wang, Y., Li, K., Gao, J., Huang, C.-H., Chen, C.-C., Ko, T.-P., Zhang, Y.,Guo, R.-T.,Oldfield, E.*. Antibacterial drug leads: DNA and enzyme multi-targeting.Journal of Medicinal Chemistry, 2015,58, 1215-1227.
76.Zheng, Y.,#Ko, T.-P.,#Yang, Y., Shao, W., *Guo, R.-T.*.Binding mode of the oxidized alpha-anomer of NAD+to RSP, a Rex-family repressor.Biochemical and Biophysical Research Communications, 2015,456, 733-736.
77.Yuan, S.-F., Wu, T.-H., Lee, H.-L., Hsieh, H.-Y., Lin, W.-L., Yang, B., Chang, C.-K., Li, Q., Gao, J., Huang, C.-H., Ho, M.-C.,*Guo, R.-T.*.Liang, P.-H.* Biochemical characterization and structural analysis of a bi-functional cellulase/xylanase fromClostridium thermocellum.The Journal of Biological Chemistry, 2015,290, 5739-5748.
78.Chen, C.-C., Huang, J.-W., Zhao, P., Ko, T.-P., Huang, C.-H., Chan, H.-C., Huang, Z., Liu, W., Cheng, Y.-S., Liu, J.-R.,Guo, R.-T.*.Structural analyses and yeast production of the β-1,3-1,4-glucanase catalytic module encoded by the licB gene ofClostridium thermocellum.Enzyme and Microbial Technology, 2015,71, 1-7.
79.Veiga-Santos, P., Li, K., Lameira, L., de Carvalho, T. M., Huang, G., Galizzi, M., Shang, N., Li, Q., Gonzalez-Pacanowska, D., Hernandez-Rodriguez, V., Benaim, G.,Guo, R.-T.,Urbina, J. A., Docampo, R., de Souza, W., Oldfield, E.*. SQ109: A new drug lead for Chagas disease.Antimicrobial Agents and Chemotherapy, 2015,59, 1950-1961.
80.Han, X.,#Chen, C.-C.,#Kuo, C.-J., Huang, C.-H., Zheng, Y., Ko, T.-P., Zhu, Z., Feng, X., Wang, K., Oldfield, E., Wang, A. H.-J., Liang, P.-H.,Guo, R.-T.,*Ma, Y.*. Crystal structures of ligand-bound octaprenyl pyrophosphate synthase fromEscherichia colireveal the catalytic and chain-length determining mechanisms.Proteins, 2015,83, 37-45.
81.Chan, H.-C.,#Feng, X.,#Ko, T.-P.#, Huang, C.-H., Hu, Y., Zheng, Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W., Crick, D. C., Liang, P.-H., Wang, A. H.-J., Oldfield, E.,*Guo, R.-T.*.Structure and inhibition of tuberculosinol synthase and decaprenyl diphosphate synthase fromMycobacterium tuberculosis.Journal of the American Chemical Society, 2014,136, 2892-2896.
82.Liu, W.,#Feng, X.,#Zheng, Y.,#Huang, C.-H., Nakano, C., Hoshino, T., Bogue, S., Ko, T.-P., Chen, C.-C., Cui, Y., Li, J., Wang, I., Hsu, S.-T. D., Oldfield, E.,*Guo, R.-T.*.Structure, function and inhibition of ent-kaurene synthase fromBradyrhizobium japonicum.Scientific Reports, 2014,4, 6214.
83.Cheng, Y.-S.,#Chen, C.-C.,#Huang, C.-H., Ko, T.-P., Luo, W., Huang, J.-W., Liu, J.-R.,Guo, R.-T.*.Structural analysis of a glycoside hydrolase family 11 xylanase fromNeocallimastix patriciarum: insights into the molecular basis of a thermophilic enzyme.The Journal of Biological Chemistry, 2014,289, 11020-11028.
84.Shang, N.,#Li, Q.,#Ko, T.-P.,#Chan, H.-C., Li, J., Zheng, Y., Huang, C.-H., Ren, F., Chen, C.-C., Zhu, Z., Galizzi, M., Li, Z. H., Rodrigues-Poveda, C. A., Gonzalez-Pacanowska, D., Veiga-Santos, P., de Carvalho, T. M., de Souza, W., Urbina, J. A., Wang, A. H.-J., Docampo, R., Li, K., Liu, Y.-L., Oldfield, E.,*Guo, R.-T.*.Squalene synthase as a target for Chagas disease therapeutics.PLoS Pathogens, 2014,10, e1004114.
85.Zheng, Y.,#Ko, T.-P.,#Sun, H., Huang, C.-H., Pei, J., Qiu, R., Wang, A. H.-J., Wiegel, J., Shao, W.,*Guo, R.-T.*.Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.Journal of Structural Biology, 2014,188, 195-204.(Cover story)
86.Peng, W.,#Ko, T.-P.,#Yang, Y., Zheng, Y., Chen, C.-C., Zhu, Z., Huang, C.-H., Zeng, Y.-F., Huang, J.-W., Wang, A. H.-J.-J., Liu, J.-R.,*Guo, R.-T.*.Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD fromClonostachys rosea. RSC Advances, 2014, 4, 62321-62325.
87.Zhu, Z.,#He, M.,#Huang, C.-H., Ko, T.-P., Zeng, Y.-F., Huang, Y.-N., Jia, S., Lu, F., Liu, J.-R.,Guo, R.-T.*.Crystallization and preliminary X-ray diffraction analysis of a novel beta-L-arabinofuranosidase (HypBA1) fromBifidobacterium longum.Acta Crystallographica. Section F, Structural Biology Communications, 2014,70, 636-638.
88.Huang, C.-H., Zhu, Z., Cheng, Y.-S., Chan, H.-C., Ko, T.-P., Chen, C.-C., Wang, I., Ho, M.-R., Hsu, S.-T. D. -D., Zeng, Y.-F., Huang, Y.-N., Liu, J.-R.,*Guo, R.-T.*.Structure and Catalytic Mechanism of a Glycoside Hydrolase Family-127β-L-Arabinofuranosidase (HypBA1).J Bioprocess Biotech, 2014,4, 171.
89.Zhang, L.,#Zhao, P.,#Chen, C.-C., Huang, C.-H., Ko, T.-P., Zheng, Y.,Guo, R.-T.*.Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase fromClostridium thermocellum.Acta Crystallographica. Section F, Structural Biology Communications, 2014,70, 946-948.
90.Yang, Y.,#Ko, T.-P.,#Liu, L., Li, J., Huang, C.-H., Chen, J.,Guo, R.-T.,*Du, G.*. Roles of tryptophan residue and disulfide bond in the variable lid region of oxidized polyvinyl alcohol hydrolase.Biochemical and Biophysical Research Communications, 2014,452, 509-514.
91.Yang, Y.,#Ko, T.-P.,#Liu, L., Li, J., Huang, C.-H., Chan, H.-C., Ren, F., Jia, D., Wang, A. H.-J.,Guo, R.-T.,*Chen, J.,* Du, G.*. Structural insights into enzymatic degradation of oxidized polyvinyl alcohol.ChemBioChem : a European Journal of Chemical Biology, 2014,15, 1882-1886.(Cover story)
92.Xu, J.,#Ren, F.,#Huang, C.-H., Zheng, Y., Zhen, J., Sun, H., Ko, T.-P., He, M., Chen, C.-C., Chan, H.-C.,Guo, R.-T.,*Song, H.,* Ma, Y.*. Functional and structural studies of pullulanase fromAnoxybacillussp. LM18-11.Proteins, 2014,82, 1685-1693.(Cover story)
93.Wu, T.-H.,#Chen, C.-C.,#Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Huang, T.-Y.,Liu, J.-R., *Guo, R.-T.*.Improving specific activity and thermostability ofEscherichia coliphytase by structure-based rational design.Journal of Biotechnology, 2014,175, 1-6.
94.Wang, S., Nie, Y., Xu, Y.,* Ko, T.-P., Huang, C.-H., Chan, H.-C.,Guo, R.-T.,*Xiao, R. Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase fromCandida parapsilosis.Acta Crystallographica. Section F, Structural Biology Communications, 2014,70, 800-802.
95.Wang, S., Nie, Y., Xu, Y.,* Zhang, R., Ko, T.-P., Huang, C.-H., Chan, H.-C.,Guo, R.-T.,*Xiao, R. Unconserved substrate-binding sites direct the stereoselectivity of medium-chain alcohol dehydrogenase.Chemical Communications, 2014,50, 7770-7772.(Cover story)
96.Tseng, C.-W.,#Liao, C.-Y.,#Sun, Y., Peng, C.-C., Tzen, J. T.,Guo, R.-T.,*Liu, J.-R.*. Immobilization ofClostridium cellulolyticumD-psicose 3-epimerase on artificial oil bodies.Journal of Agricultural and Food Chemistry, 2014,62, 6771-6776.
97.Lv, P.,#Zhang, L.,#Luo, H., Chen, C.-C., Huang, C.-H., Peng, W., Wang, K., Ko, T.-P., Zheng, Y., Zhang, J., Yao, B.,*Guo, R.-T.*.Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase fromStreptomycessp. S9.Acta Crystallographica. Section F, Structural Biology Communications, 2014,70, 105-107.
98.Liu, W., Li, Z., Huang, C.-H.,Guo, R.-T.,Zhao, L., Zhang, D., Chen, X., Wu, Q., Zhu, D.*. Structural and mutational studies on the unusual substrate specificity of meso-diaminopimelate dehydrogenase fromSymbiobacterium thermophilum.ChemBioChem : a European Journal of Chemical Biology, 2014,15, 217-222.(Cover story)
99.Li, K., Schurig-Briccio, L. A., Feng, X., Upadhyay, A., Pujari, V., Lechartier, B., Fontes, F. L., Yang, H., Rao, G., Zhu, W., Gulati, A., No, J.-H., Cintra, G., Bogue, S., Liu, Y.-L., Molohon, K., Orlean, P., Mitchell, D. A., Freitas-Junior, L., Ren, F., Sun, H., Jiang, T., Li, Y.,Guo, R.-T.,Cole, S. T., Gennis, R. B., Crick, D. C., Oldfield, E.*. Multitarget drug discovery for tuberculosis and other infectious diseases.Journal of Medicinal Chemistry, 2014,57, 3126-3139.
100.Huang, J.-W.,#Chen, C.-C.,#Huang, C.-H., Huang, T.-Y., Wu, T.-H., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y.,Liu, J.-R.,*Guo, R.-T.*.Improving the specific activity of beta-mannanase fromAspergillus nigerBK01 by structure-based rational design.Biochimica et Biophysica Acta, 2014,1844, 663-669.(Cover story)
101.He, M.,#Zheng, Y.,#Huang, C.-H., Qian, G., Xiao, X., Ko, T.-P., Shao, W.,Guo, R.-T.*.Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) fromThermotoga maritima.Acta Crystallographica. Section F, Structural Biology Communications, 2014,70, 1563-1565.
102.Feng, X.,#Hu, Y.,#Zheng, Y.,#Zhu, W., Li, K., Huang, C.-H., Ko, T.-P., Ren, F., Chan, H.-C., Nega, M., Bogue, S., Lopez, D., Kolter, R., Gotz, F.,Guo, R.-T.,*Oldfield, E.*. Structural and functional analysis ofBacillus subtilisYisP reveals a role of its product in biofilm production.Chemistry & Biology, 2014,21, 1557-1563.
103.Cheng, Y.-S.,#Huang, C.-H.,#Chen, C.-C., Huang, T.-Y., Ko, T.-P., Huang, J.-W., Wu, T.-H.,Liu, J.-R.,*Guo, R.-T.*.Structural and mutagenetic analyses of a 1,3-1,4-beta-glucanase fromPaecilomyces thermophila.Biochimica et Biophysica Acta, 2014,1844, 366-373.(Cover story)
104.Chen, C.-C.,#Luo, H.,#Han, X.,#Lv, P., Ko, T.-P., Peng, W., Huang, C.-H., Wang, K., Gao, J., Zheng, Y., Yang, Y., Zhang, J., Yao, B.,*Guo, R.-T.*.Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability.Journal of Biotechnology, 2014,189, 175-182.
105.Bai, W., Zhou, C., Xue, Y., Huang, C.-H.,Guo, R.-T.,Ma, Y.*. Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilicBacillussp. SN5 and improvement of its thermal performance by introducing arginines substitutions.Biotechnology Letters, 2014,36, 1495-1501.
106.Ren, F.,#Feng, X.,#Ko, T.-P.,#Huang, C.-H., Hu, Y., Chan, H.-C., Liu, Y.-L., Wang, K., Chen, C.-C., Pang, X., He, M., Li, Y., Oldfield, E.,*Guo, R.-T.*.Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB fromBacillus subtilisandStaphylococcus aureus.ChemBioChem : a European Journal of Chemical Biology, 2013,14, 195-199.(Cover story)
107.Luo, W.,#Huang, J.-W.,#Huang, C.-H., Huang, T.-Y., Chan, H.-C., Liu, J.-R.,Guo, R.-T.,Chen, C.-C.*. Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase fromAspergillus nigerBK01.Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2013,69, 1100-1102.
108.Li, X.,#Han, X.,#Ko, T.-P., Chen, C.-C., Zhu, Z., Hua, E.,Guo, R.-T.,Huang, C.-H.*. Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase fromEscherichia coli.Acta crystallographica. Section F, Structural Biology and Crystallization Communications, 2013,69, 328-331.
109.Jiang, T.,#Chan, H.-C.,#Huang, C.-H., Ko, T.-P., Huang, T.-Y.,Liu, J.-R.,Guo, R.-T.*.Substrate binding to a GH131 beta-glucanase catalytic domain fromPodospora anserina.Biochemical and Biophysical Research Communications, 2013,438, 193-197.
110.Hu, Y., Jia, S., Ren, F., Huang, C.-H., Ko, T.-P., Mitchell, D. A.,Guo, R.-T.,Zheng, Y.*. Crystallization and preliminary X-ray diffraction analysis of YisP protein fromBacillus subtilissubsp. subtilis strain 168.Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2013,69, 77-79.
111.Han, X.,#Gao, J.,#Shang, N.,#Huang, C.-H., Ko, T.-P., Chen, C.-C., Chan, H.-C., Cheng, Y.-S., Zhu, Z., Wiegel, J., Luo, W.,*Guo, R.-T.,*Ma, Y.*. Structural and functional analyses of catalytic domain of GH10 xylanase fromThermoanaerobacterium saccharolyticumJW/SL-YS485.Proteins, 2013,81, 1256-1265.(Cover story)
112.Chang, T.-H.,#Chang, S. J.,#Hsieh, F. L., Ko, T.-P., Lin, C.-T., Ho, M.-R., Wang, I., Hsu, S.-T. D.,Guo, R.-T.,Chang, W., Wang, A. H.-J.*. Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein.PLoS Pathogens, 2013,9, e1003563.
113.Ren, F.,#Ko, T.-P.,#Feng, X., Huang, C.-H., Chan, H.-C., Hu, Y., Wang, K., Ma, Y., Liang, P.-H., Wang, A. H.-J., Oldfield, E.,*Guo, R.-T.*.Insights into the mechanism of the antibiotic-synthesizing enzyme MoeO5 from crystal structures of different complexes.Angewandte Chemie International Edition,2012,51, 4157-4160.(Cover story)
114.Zheng, Y., Huang, C.-H., Liu, W., Ko, T.-P., Xue, Y., Zhou, C.,Guo, R.-T.,Ma, Y.*. Crystal structure and substrate-binding mode of a novel pectate lyase from alkaliphilicBacillussp. N16-5.Biochemical and Biophysical Research Communications, 2012,420, 269-274.
115.Liu, W., Sun, Y., Ko, T.-P., Wiegel, J., Shao, W., Lu, F.,Guo, R.-T.,Huang, C.-H.*. Crystallization and preliminary X-ray diffraction analysis of a novel GH120 beta-xylosidase (XylC) fromThermoanaerobacterium saccharolyticumJW/SL-YS485.Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2012,68, 914-916.
116.Huang, J.-W., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Chen, C.-C., Ma, Y., Zheng, Y., Huang, C.-H., Zou, P.,Liu, J.-R.,*Guo, R.-T.*.Rational design to improve thermostability and specific activity of the truncatedFibrobacter succinogenes1,3-1,4-beta-D-glucanase.Applied Microbiology and Biotechnology, 2012,94, 111-121.
117.Huang, C.-H.,#Sun, Y.,#Ko, T.-P., Chen, C.-C., Zheng, Y., Chan, H.-C., Pang, X., Wiegel, J.,* Shao, W.,*Guo, R.-T.*.The substrate/product-binding modes of a novel GH120 beta-xylosidase (XylC) fromThermoanaerobacterium saccharolyticumJW/SL-YS485.The Biochemical Journal, 2012,448, 401-407.
118.Cheng, Y.-S., Ko, T.-P., Huang, J.-W., Wu, T.-H., Lin, C.-Y., Luo, W., Li, Q., Ma, Y., Huang, C.-H., Wang, A. H.-J.,Liu, J.-R.,*Guo, R.-T.*.Enhanced activity ofThermotoga maritimacellulase 12A by mutating a unique surface loop.Applied Microbiology and Biotechnology, 2012,95, 661-669.
119.Chang, K.-M., Chen, S.-H., Kuo, C.-J., Chang, C.-K.,Guo, R.-T.,Yang, J.M., Liang, P.-H.*. Roles of amino acids in theEscherichia colioctaprenyl diphosphate synthase active site probed by structure-guided site-directed mutagenesis.Biochemistry, 2012,51, 3412-3419.
120.Chan, H.-C.,#Zhu, Y.,#Hu, Y., Ko, T.-P., Huang, C.-H., Ren, F., Chen, C.-C., Ma, Y.,Guo, R.-T.,*Sun, Y.*. Crystal structures of D-psicose 3-epimerase fromClostridium cellulolyticumH10 and its complex with ketohexose sugars.Protein & Cell, 2012,3, 123-131.(Cover story)
121.Pai, C. K. Wang, H. T., Guo, R.-T., Liu, J.-R.*. The construction of bifunctional fusion xylanolytic enzymes and the prediction of optimum reaction conditions for the enzyme activity.BioResources,2012,7, 5647-5665.
122.Zeng, Y.-F.,#Ko, T.-P.,#Lai, H.-L., Cheng, Y.-S., Wu, T.-H., Ma, Y., Chen, C.-C., Yang, C. S., Cheng, K. J., Huang, C.-H.,Guo, R.-T.,*Liu, J.-R.*. Crystal structures ofBacillusalkaline phytase in complex with divalent metal ions and inositol hexasulfate.Journal of Molecular Biology, 2011,409, 214-224.(Cover story)
123.Wu, T.-H.,#Huang, C.-H.,#Ko, T.-P., Lai, H.-L., Ma, Y., Chen, C.-C., Cheng, Y.-S., Liu, J.-R.,*Guo, R.-T.*.Diverse substrate recognition mechanism revealed byThermotoga maritimaCel5A structures in complex with cellotetraose, cellobiose and mannotriose.Biochimica et Biophysica Acta, 2011,1814, 1832-1840.(Cover story)
124.Tseng, C.-W.,#Ko, T.-P.,#Guo, R.-T.,#Huang, J.-W., Wang, H.-C., Huang, C.-H., Cheng, Y.-S., Wang, A. H.-J., Liu, J.-R.*. Substrate binding of a GH5 endoglucanase from the ruminal fungusPiromyces rhizinflata.Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2011,67, 1189-1194.(Cover story)
125.Cheng, Y.-S., Ko, T.-P., Wu, T.-H., Ma, Y., Huang, C.-H., Lai, H.-L., Wang, A. H.-J., Liu, J.-R.,*Guo, R.-T.*.Crystal structure and substrate-binding mode of cellulase 12A fromThermotoga maritima.Proteins, 2011,79, 1193-1204.(Cover story)
126.Zhang, Y., Cao, R., Yin, F., Hudock, M. P.,Guo, R.-T.,Krysiak, K., Mukherjee, S., Gao, Y.-G., Robinson, H., Song, Y., No, J.-H., Bergan, K., Leon, A., Cass, L., Goddard, A., Chang, T.-K., Lin, F.-Y., Van Beek, E., Papapoulos, S., Wang, A. H.-J., Kubo, T., Ochi, M., Mukkamala, D., Oldfield, E.*. Lipophilic bisphosphonates as dual farnesyl/geranylgeranyl diphosphate synthase inhibitors: an X-ray and NMR investigation.Journal of the American Chemical Society, 2009,131, 5153-5162.
127.Guo, R.-T.,#Chong, Y.-E.,#Guo, M., Yang, X.-L.*. Crystal structures and biochemical analyses suggest a unique mechanism and role for human glycyl-tRNA synthetase in Ap4A homeostasis.The Journal of Biological Chemistry, 2009,284, 28968-28976.
128.Chen, C.-K., Lee, G.-C., Ko, T.-P.,Guo, R.-T.,Huang, L.-M., Liu, H.-J., Ho, Y.-F., Shaw, J.-F., Wang, A. H.-J.*. Structure of the alkalohyper thermophilicArchaeoglobus fulgiduslipase contains a unique C-terminal domain essential for long-chain substrate binding.Journal of Molecular Biology, 2009,390, 672-685.
129.Wu, Y.-H., Ko, T.-P.,Guo, R.-T.,Hu, S.-M.,Chuang, L.-M., Wang, A. H.-J.*. Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2.Structure, 2008,16, 1714-1723.
130.Kuo, C.-J.,Guo, R.-T.,Lu, I.-L., Liu, H.-G., Wu, S.-Y., Ko, T.-P.,Wang, A. H.-J., Liang, P.-H.*. Structure-based inhibitors exhibit differential activities againstHelicobacter pyloriandEscherichia coliundecaprenyl pyrophosphate synthases.Journal of Biomedicine & Biotechnology, 2008, 841312.
131.Jeng, W.-Y., Ko, T.-P., Liu, C.-I.,Guo, R.-T.,Liu, C.-L.,Shr, H.-L., Wang, A. H.-J.*. Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation inStaphylococcus epidermidis.Nucleic Acids Research, 2008,36, 1567-1577.
132.Cao, R., Chen, C.-K. C.,Guo, R.-T.,Wang, A. H.-J.,* Oldfield, E.*. Structures of a potent phenylalkyl bisphosphonate inhibitor bound to farnesyl and geranylgeranyl diphosphate synthases.Proteins, 2008,73, 431-439.
133.Chen, K.-M. C., Hudock, M. P., Zhang, Y.,Guo, R.-T.,Cao, R., No, J.-H., Liang, P.-H., Ko, T.-P., Chang, T.-H., Chang, S.-C., Song, Y., Axelson, J., Kumar, A.,Wang, A. H.-J., Oldfield, E.*. Inhibition of geranylgeranyl diphosphate synthase by bisphosphonates: a crystallographic and computational investigation.Journal of Medicinal Chemistry, 2008,51, 5594-5607.
134.Guo, R.-T.,#Cao, R.,#Liang, P.-H., Ko, T.-P., Chang, T.-H., Hudock, M. P., Jeng, W.-Y., Chen, C.-K., Zhang, Y., Song, Y., Kuo, C.-J., Yin, F., Oldfield, E., Wang, A. H.-J.*. Bisphosphonates target multiple sites in bothcis- andtrans-prenyltransferases.Proceedings of the National Academy of Sciences of the United States of America, 2007,104, 10022-10027.
135.Chang, T.-H.,Guo, R.-T.,Ko, T.-P.,Wang, A. H.-J.,* Liang, P.-H.*. Crystal structure of type-III geranylgeranyl pyrophosphate synthase fromSaccharomyces cerevisiaeand the mechanism of product chain length determination.The Journal of Biological Chemistry, 2006,281, 14991-15000.
136.Sun, H.-Y., Ko, T.-P., Kuo, C.-J.,Guo, R.-T.,Chou, C.-C.,Liang, P.-H.,* Wang, A. H.-J.*. Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeonSulfolobus solfataricusdisplays asymmetric subunit structures.Journal of Bacteriology, 2005,187, 8137-8148.
137.Guo, R.-T.,#Ko, T.-P.,#Chen, A. P., Kuo, C.-J., Wang, A. H.-J.,* Liang, P.-H.*. Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis.The Journal of Biological Chemistry, 2005,280, 20762-20774.
138.Guo, R.-T.,#Kuo, C.-J.,#Ko, T.-P., Chou, C.-C.,Liang, P.-H.,* Wang, A. H.-J.*. A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products.Biochemistry, 2004,43, 7678-7686.
139.Guo, R.-T.,Kuo, C.-J., Chou, C.-C., Ko, T.-P., Shr, H.-L.,Liang, P.-H.,* Wang, A. H.-J.*. Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilicThermotoga maritimaand mechanism of product chain length determination.The Journal of Biological Chemistry, 2004,279, 4903-4912.
140.Chu, H.-M.,#Guo, R.-T.,#Lin, T. W., Chou, C.-C., Shr, H.-L., Lai, H.-L., Tang, T.-Y., Cheng, K.-J., Selinger, B. L., Wang, A. H.-J.*. Structures ofSelenomonas ruminantiumphytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis.Structure, 2004,12, 2015-2024.
141.Guo, R.-T.,#Ko, T.-P.,#Chou, C.-C., Shr, H.-L., Chu, H.-M., Tsai, Y.-H., Liang, P.-H., Wang, A. H.-J.*. Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals fromThermotoga maritimaandEscherichia coli.Acta Crystallographica. Section D, Biological Crystallography, 2003,59, 2265-2268.
142.Guo, R.-T.,Chou, L.-J., Chen, Y.-C., Chen, C.-Y., Pari, K., Jen, C. J., Lo, S. J., Huang, S.-L., Lee, C.-Y.,Chang, T.-W., Chuang, W.-J.*. Expression inPichia pastorisand characterization by circular dichroism and NMR of rhodostomin.Proteins, 2001,43, 499-508.
六、已授权专利
1.郭瑞庭，郑雅珊，吴姿慧，黄建文，赖惠琳，林正言。一种纤维素酶及其应用，授权号：ZL2011100426000，中国专利。
2.郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言。酶活性及热稳定性被提高的葡聚糖酶，授权号：ZL2011100596815，中国专利。
3.郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言，黄婷沅。提升酶活性之木聚糖酶，ZL2013101344011，中国专利。
4.郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言，黄婷沅。酶活性提高的甘露聚糖酶，授权号：ZL201310135208X，中国专利。
5.郭瑞庭，陈纯琪，吴姿慧，郑雅珊，黄建文，赖惠琳，林正言，黄婷沅。具有提升的酶活性的植酸酶，授权号：CN104342418B，中国专利。
6.郭瑞庭，陈纯琪，吴姿慧，郑雅珊，黄建文，赖惠琳，林正言，黄婷沅。具有提升的酶活性的植酸酶，授权号：CN106399273B，中国专利。
7.Guo, R.-T.,Zheng, Y., Xu, Z. , Chen, C.-C., Peng, W., Liu, W., Liu, J.-R., Ma, Y. Lactone hydrolase and method of degrading alpha-zearalenol using the same，授权号：US10316303B2，美国专利。
8.Guo, R.-T.,Cheng, Y.-S.,Wu, T.-H.,Huang, J.-W.,Lai, H.-L.,Lin, C.-Y.，Thermostable cellulase having increased enzyme activity，授权号US8137945B1，美国专利。
9.Guo, R.-T.,Huang, J.-W.,Cheng, Y.-S.,Wu, T.-H.,Lai, H.-L.,Lin C.-Y.，Glucanase having increased enzyme activity and thermostability，授权号US8192974B1，美国专利。
10.Guo, R.-T.,Cheng, Y.-S.,Huang, J.-W.,Wu, T.-H.,Lai, H.-L.,Lin, C.-Y.,Huang, T.-Y.，Xylanase having improved enzymatic activity，授权号US8722382B1，美国专利。
11.Guo, R.-T.,Huang, J.-W.,Cheng, Y.-S.,Wu, T.-H.,Lai, H.-L.,Lin, C.-Y., Huang, T.-Y.，Beta-mannanase having improved enzymatic activity，授权号US8865444B1，美国专利。
12.Guo, R.-T.,Huang, J.-W., Cheng, Y.-S., Wu, T.-H., Lai, H.-L., Lin C.-Y., Huang T.-Y. β-mannanase having improved production yield and enzymatic activity，授权号US8999695B1，美国专利。
13.Guo, R.-T.,Wu, T.-H.,Cheng, Y.-S.,Huang, J.-W.,Lai, H.-L.,Lin C.-Y., Huang T.-Y. Phytase having improved enzymatic activity，授权号US9194010B2，美国专利。
14.Chen, C.-C.,Huang, J.-W., Dai, L., Yu, X., Chen, C.-Y., Wu, S.,Zhan, Z., Zhang, L., Zhai, C.,Ma, L.,Guo, R.-T.Cellulase having improved enzymatic activity，授权号：US10479985B1，美国专利。
15.Guo, R.-T.,Chen, C.-C.,Cheng, Y.-S.,Huang, J.-W.,Wu, T.-H.,Lai, H.-L.,Lin, C.-Y.,Ko, T.-Y.Cellulase having improved enzymatic activity，授权号：US9809807B1，美国专利。
16.Wu, T.-H.,Cheng, Y.-S.,Lai, H.-L.,Ke, T.-Y.,Huang, J.-W.,Chen, C.-C.,Guo, R.-T.Phytase having improved thermostability，授权号：US10011822B2，美国专利。
17.Cheng, Y.-S.,Wu, T.-H.,Lin, C.-Y.,Lai, H.-L.,Zheng, C.-B.,Huang, T.-Y.,Lin, I.-H.,Huang, J.-W.,Chen, C.-C.,Guo, R.-T.Glucose oxidase having improved thermostability，授权号：US10233430B1，美国专利。
18.Chen, C.-C.,Wang, T.,Huang, J.-W.,Guo, R.-T.Lysozyme having improved enzymatic activity，授权号：US10323237B2，美国专利。
19.Cheng, Y.-S.,Wu, T.-H.,Lin, C.-Y., Lai, H.-L., Zheng, C.-B., Huang, T.-Y.,Lin, I.-H.,Huang, J.-W.,Chen, C.-C.,Guo, R.-T.Xylosidase having improved enzymatic activity，授权号：US10385346B2，美国专利。
七、获奖与荣誉称号
1.2020年，“湖北省创新群体”(群体负责人)
2.2019年，“湖北省省级人才入选者”
3.2019年，“天津市自然科学二等奖”(第一完成人)
4.2018年，“国家级人才入选者”
5.2017年，“部级人才入选者”
6.2015年，“天津市131第一层次人才培养工程”
7.2012年，“天津市131第二层次人才培养工程”
8.2012年，“2011年科学中国人年度人物”
9.2010年，“中国科学院人才计划入选者”，择优支持
10.2010年，“天津市市级人才计划入选者”，为天津市特聘教授
2021年以湖北大学图书馆为背景发表的P450综述文章
获选的其中25篇封面文章
